Progress on the DNA project with Lukas. These YOYO-1 stained double stranded DNA molecules are finally all stretching out nicely using our own variation on shear combing. Low power 488 nm excitation seems to be doing the trick well. Further optimization will hopefully allow better isolation of single molecules and improved localization precision. 

First big success on our new CHM2990 undergraduate research project. Kudos to Lukas for these sub-diffraction images of ATTO labelled silica spheres. We were a bit worried when he told us the 100 nm spheres he had labelled measured at 80 nm across but EM confirmed the heterogeneity of the sample. These could make a handy material for all sorts of future applications! [Scale bars © 1 micron and (D) 100 nm.]