Further success on the Spheres project which is now rolling into a summer undergraduate project. Using similar protocols developed for the 100 nm spheres, Lukas has labelled a 20 nm batch with ATTO 647N dyes and grabbed some very nice single molecule images. EM once again confirms the heterogeneity of the spheres. Localization precision on the setup is exceeding expectations! [Scale bar (D) 100 nm.]

First big success on our new CHM2990 undergraduate research project. Kudos to Lukas for these sub-diffraction images of ATTO labelled silica spheres. We were a bit worried when he told us the 100 nm spheres he had labelled measured at 80 nm across but EM confirmed the heterogeneity of the sample. These could make a handy material for all sorts of future applications! [Scale bars © 1 micron and (D) 100 nm.]