Optimization of fixation protocols and characterization of fixation artefacts for single molecule super resolution microscopy continues! Among the many images gathered was this one containing one of the most confused microtubules we’ve ever seen… Or maybe we’ve just been looking at microtubules for too long.

More zoomed in microtubule images. These ones were fixed using methanol and once again some interesting looking kinks in the structure manifested. Still not perfect but we’re getting there!