Sometimes all it takes is a couple of minutes and a tweak of the setup to get the very best blinking! The red laser and the Q-TIRF x-y stage were both used extensively today for ongoing experiments in collaboration with Dr Gregory Moseley into the effects of the Rabies P protein on microtubule architecture. 

The Scope as it now stands. Ready to run three color simultaneous excitation and two channel detection. Simple as that. 

Our first look at mitochondria and a very successful switch to a ATTO 488, a blue fluorophore to test out the new blue laser. 

A great new test sample for benchmarking the setup has been helpfully provided by Dr Georg Ramm. Single molecule imaging of LC3 reveals both a high level of homogeneous background as well as allowing visualization of autophagosomes in starved cells. Very cool.