A new single molecule super resolution project has been thought up to run alongside FCS measurements of Nile Red stained liposomes. As shown in 2006 by Hochstrasser, the diffusion of Nile Red in and out of lipid bilayers allows for single molecule localizations as the diffusing molecule only fluoresces from within the non-polar environment of the lipid tails. Preliminary data of sub-diffraction liposomes immobilised on glass shows the potential of such a method. Future work will optimize liposomes and lipid bilayer preparation for SMLM imaging, as well as move the technique into cell samples.