Further success on the Spheres project which is now rolling into a summer undergraduate project. Using similar protocols developed for the 100 nm spheres, Lukas has labelled a 20 nm batch with ATTO 647N dyes and grabbed some very nice single molecule images. EM once again confirms the heterogeneity of the spheres. Localization precision on the setup is exceeding expectations! [Scale bar (D) 100 nm.]